Further Studies on the Biosynthesis of Pseudoisoeugenols in Tissue Cultures of Pimpinella anisum
نویسندگان
چکیده
Tissue cultures o f Pimpinella anisum and P. major were used to study the biosynthesis o f pseudoisoeugenols. The putative precursors were labelled with 13C with one exception that was labelled with i4C. The incorporation o f the label was controlled by 13C N M R and liquid scintil lation, respectively. The biosynthetic sequence found was L-phenylalanine, /-cinnamic acid, /?-coumaric acid, / 7-hydroxycoumaric alcohol, /»-methoxycoumaric alcohol and anethol. The incorporation rates ranged from 0.5% to 25%.
منابع مشابه
Production and Accumulation of Phenylpropanoids in Tissue and Organ Cultures of Pimpinella anisum
Recently we reported the comparative studies on the production and accumulation of phenylpropanoids in plants and in cell cultures of anise [1, 2]. It was shown that cell cultures did not produce anethole, the main constituent in the fruit oil of anise, but instead regularly epoxy-pseudoisoeugenol(2-methylbutyrate). We first identified this unusually substituted phenylpropanoid in the root of P...
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* Institut für Pharmazeutische Biologie der Universität Heidelberg, Im Neuenheimer Feld 364, D-6900 Heidelberg. Bundesrepublik Deutschland ** Organisch-Chemisches Institut der Universität Heidelberg, Im Neuenheimer Feld 270, D-6900 Heidelberg. Bundesrepublik Deutschland Z. Naturforsch. 43c, 328-336 (1988); received January 20, 1988 Pimpinella anisum, Umbelliferae, Pseudoisoeugenols, Tissue Cult...
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